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The complexity of the base-calling discretization process results in reads of widely varying quality within and across sequence samples. This variation in processing quality results in infrequent but systematic errors that we have found to mislead downstream analysis of the discretized sequence read data. For instance, a central goal of the Genomes Project is to quantify across-sample variation at the single nucleotide level.

At this resolution, small error rates in sequencing prove significant, especially for rare variants. Sec-gen sequencing is a relatively new technology for which potential biases and sources of obscuring variation are not yet fully understood. Therefore, modeling and quantifying the uncertainty inherent in the generation of sequence reads is of utmost importance. Model parameters have a straightforward interpretation in terms of the chemistry of base-calling allowing for informative and easily interpretable metrics that capture the variability in. Body fluid identification of blood, saliva and semen using second generation sequencing of micro-RNA.

We report a new second generation sequencing method for identification micro-RNA miRNA that can be used to identify body fluids and tissues. Principal component analysis of 10 miRNAs with high expression in 16 samples of blood, saliva and semen showed clear differences in the expression of mi Given that Roche has announced that the platforms will no longer be supported from , focus should now be shifted to competing SGS There are currently several challenges faced with amplicon-based SGS STR typing in forensic genetics, including current lengths of amplicons for CE-typing and lack of uniform data analysis between Full profiles and concordant results between replicate SGS runs and CE-typing were observed for all control samples.

Full profiles were seen with DNA input down to 50pg, with the exception of a single locus Analysis of quality raw data of second generation sequencers with Quality Assessment Software. Second generation technologies have advantages over Sanger; however, they have resulted in new challenges for the genome construction process, especially because of the small size of the reads, despite the high degree of coverage.

Independent of the program chosen for the construction process, DNA sequences are superimposed, based on identity, to extend the reads, generating contigs; mismatches indicate a lack of homology and are not included. This process improves our confidence in the sequences that are generated.

We developed Quality Assessment Software, with which one can review graphs showing the distribution of quality values from the sequencing reads. This software allow us to adopt more stringent quality standards for sequence data, based on quality-graph analysis and estimated coverage after applying the quality filter, providing acceptable sequence coverage for genome construction from short reads. Quality filtering is a fundamental step in the process of constructing genomes, as it reduces the frequency of incorrect alignments that are caused by measuring errors, which can occur during the construction process due to the size of the reads, provoking misassemblies.

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Application of quality filters to sequence data, using the software Quality Assessment, along with graphing analyses, provided greater precision in the definition of cutoff parameters, which increased the accuracy of genome construction. Dokeos used to be one of the leading open source learning platforms. It is well known for the wide range of functions it offers and especially for its ease of use. In many ways it is however a typical first generation learning management system LMS consisting of a set of loosely integrated tools. The original design of this LMS has a number of serious drawbacks and limitations.

Because of this it was decided to opt for a completely new system that would use state of the art development tech Transcriptome sequencing of lentil based on second-generation technology permits large-scale unigene assembly and SSR marker discovery. In terms of genomic resources, lentil is relatively underdeveloped, in comparison to other Fabaceae species, with limited available data. There is hence a significant need to enhance such resources in order to identify novel genes and alleles for molecular breeding to increase crop productivity and quality.

De novo assembly generated a total of 15, contigs and 68, singletons. The complete unigene set was sequence -analysed against genome drafts of the model legume species Medicago truncatula and Arabidopsis thaliana to identify 12,, and 7, unique matches, respectively. When compared to the genome of Glycine max, a total of 20, unique hits were observed corresponding to c.

A total of 25, lentil unigenes were subsequently annoated from GenBank. Simple sequence repeat SSR-containing ESTs were identified from consensus sequences and a total of 2, primer pairs were designed. A subset of EST-SSR markers was screened for validation across a panel 12 cultivated lentil genotypes and one wild relative species. A total of primer pairs obtained successful amplification, of which Conclusions A substantial collection of ESTs has been developed from sequence analysis of lentil genotypes using second-generation technology, permitting unigene definition across a broad range of functional categories.

As well as providing resources for functional genomics studies, the unigene set has permitted significant enhancement of the number of publicly-available molecular genetic markers as tools for improvement of this species. Modelling noise in second generation sequencing forensic genetics STR data using a one-inflated zero-truncated negative binomial model. We present a model fitting the distribution of non-systematic errors in STR second generation sequencing , SGS, analysis. The model fits the distribution of non-systematic errors, i. The model is a two component model Unsatisfactory clinical outcomes of second-generation mobile bearing floating platform total knee arthroplasty: The study retrospectively enrolled patients who received the UC type fixed bearing insert group 1 and 95 patients who received the floating platform MB insert group 2 during the period from August to June All surgery was performed using the navigation-assisted gap balancing technique.

For strict assessment of gap measurements, the offset-type-force-controlled-spreader-system was used. For statistical analysis, paired sample t tests were used. A p value less than 0. There were two cases of insert breakage, which required bearing exchange. Other patients who underwent surgery with second-generation MB floating platform were encouraged to avoid high knee flexion activities, resulting in lower clinical performance. Ultra-hydrophilic stent platforms promote early vascular healing and minimise late tissue response: Simple surface modifications can enhance coronary stent performance.

Ultra-hydrophilic surface UHS treatment of contemporary bare metal stents BMS was assessed in vivo to verify whether such stents can provide long-term efficacy comparable to second-generation drug-eluting stents DES while promoting healing comparably to BMS. A thirty-day and a day porcine coronary model were used to characterise late tissue response. Three-day porcine coronary and seven-day rabbit iliac models were used for early healing assessment.

In porcine coronary arteries, hydrophilic treatment reduced intimal hyperplasia relative to the BMS and corresponding DES platforms 1. The complete mitochondrial genome of the Chinstrap penguin Pygoscelis antarcticus was sequenced and compared with other penguin mitogenomes. The genome is 15, bp in length with the number and order of protein coding genes and RNAs being very similar to that of other known penguin mitogenomes.

Divergence at nonsynonymous nucleotide positions was found to be up to 23 times less than that observed in synonymous positions of protein coding genes, suggesting high selection constraints. The complete mitogenome data will be useful for genetic and evolutionary studies of penguins.


Second generation registry framework. Information management systems are essential to capture data be it for public health and human disease, sustainable agriculture, or plant and animal biosecurity. In public health, the term patient registry is often used to describe information management systems that are used to record and track phenotypic data of patients. Appropriate design, implementation and deployment of patient registries enables rapid decision making and ongoing data mining ultimately leading to improved patient outcomes. A major bottleneck encountered is the static nature of these registries.

That is, software developers are required to work with stakeholders to determine requirements, design the system, implement the required data fields and functionality for each patient registry. Additionally, software developer time is required for ongoing maintenance and customisation.

It is desirable to deploy a sophisticated registry framework that can allow scientists and registry curators possessing standard computing skills to dynamically construct a complete patient registry from scratch and customise it for their specific needs with little or no need to engage a software developer at any stage. This paper introduces our second generation open source registry framework which builds on our previous rare disease registry framework RDRF.

This second generation RDRF is a new approach as it empowers registry administrators to construct one or more patient registries without software developer effort. New data elements for a diverse range of phenotypic and genotypic measurements can be defined at any time. Defined data elements can then be utilised in any of the created registries. Fine grained, multi-level user and workgroup access can be applied to each data element to ensure appropriate access and data privacy. We introduce the concept of derived data elements to assist the data element standards communities on how they might be best categorised.

We introduce the second generation RDRF that. A second generation framework for the analysis of microsatellites in expressed sequence tags and the development of EST-SSR markers for a conifer, Cryptomeria japonica. Full Text Available Abstract Background Microsatellites or simple sequence repeats SSRs in expressed sequence tags ESTs are useful resources for genome analysis because of their abundance, functionality and polymorphism. The advent of commercial second generation sequencing machines has lead to new strategies for developing EST-SSR markers, necessitating the development of bioinformatic framework that can keep pace with the increasing quality and quantity of sequence data produced.

Results We collected , sequence reads by Sanger sequencing and 1,, by pyrosequencing. After trimming contaminant and low quality sequences , , Sanger and 1,, pyrosequencing reads were passed to the MIRA assembler, generating 81, contigs that were analysed for SSRs. Markers resulting from both pipelines showed no differences in PCR success rate and polymorphisms, but PCR success and polymorphism were significantly affected by the expected PCR product size.

Jake Shannon (Author of Say Uncle!)

Background Microsatellites or simple sequence repeats SSRs in expressed sequence tags ESTs are useful resources for genome analysis because of their abundance, functionality and polymorphism. Automated DNA sequencing instruments embody an elegant interplay among chemistry, engineering, software, and molecular biology and have built upon Sanger's founding discovery of dideoxynucleotide sequencing to perform once-unfathomable tasks.

Combined with innovative physical mapping approaches that helped to establish long-range relationships between cloned stretches of genomic DNA, fluorescent DNA sequencers produced reference genome sequences for model organisms and for the reference human genome. New types of sequencing instruments that permit amazing acceleration of data-collection rates for DNA sequencing have been developed. The ability to generate genome-scale data sets is now transforming the nature of biological inquiry. Here, I provide an historical perspective of the field, focusing on the fundamental developments that predated the advent of next-generation sequencing instruments and providing information about how these instruments work, their application to biological research, and the newest types of sequencers that can extract data from single DNA molecules.

The second-generation tools are growing in popularity…. The discovery of high temperature superconductors HTS in by two IBM scientists led to an unprecedented explosion of research and development efforts world-wide because of the significant potential for practical applications offered by these materials. However, the early euphoria created by the exciting prospects was dampened by the daunting task of fabricating these materials into useful forms with acceptable superconducting properties.

Progress towards this goal has been hindered by many intrinsic materials problems, such as weak-links, flux-creep, and poor mechanical properties. Three methods were invented to produce flexible metallic substrates, which were also crystallographically biaxially textured, and resembled a long, mosaic single crystal. The third method invented is calle Full Text Available Direct sequencing of total plant DNA using next generation sequencing technologies generates a whole chloroplast genome sequence that has the potential to provide a barcode for use in plant and food identification.

Advances in DNA sequencing platforms may make this an attractive approach for routine plant identification. The HiSeq Illumina and Ion Torrent Life Technology sequencing platforms were used to sequence total DNA from rice to identify polymorphisms in the whole chloroplast genome sequence of a wild rice plant relative to cultivated rice cv. Consensus chloroplast sequences were produced by mapping sequence reads to the reference rice chloroplast genome or by de novo assembly and mapping of the resulting contigs to the reference sequence.

A total of polymorphisms SNPs and indels between the wild and cultivated rice chloroplasts were predicted by these different sequencing and analysis methods. Of these, a total of polymorphisms including 90 SNPs were predicted by both platforms. Indels were more variable with different sequencing methods, with almost all discrepancies found in homopolymers. The methods should be suitable for routine barcoding using appropriate combinations of sequencing platform and data analysis. Additionally, we got an average sequence depth for the genome of for the Iontorrent PGM and for the FLX platform making the mapping suitable for single nucleotide variant SNV detection.

Next Generation Sequencing NGS is becoming more adopted into viral research and will be the preferred technology in the years to come. The second generation biofuels from the biomass. The author takes stock on the second generation biofuels in the world, the recent technologies, their advantages, the research programs and the economical and environmental impacts of the biofuels development.

The study indicates that while second generation biofuels could significantly contribute to the future energy supply mix, cost is a major barrier to its commercial production in the near to medium term. Depending upon type of biofuels, feedstock prices and conversion costs, the cost of cellulosic ethanol is found to be two to three times higher than the current price of gasoline on an energy equivalent basis.

THE 4 RISKS YOU NEED TO KNOW: Global Threats That Will Impact Your Wealth

The median cost across the studies reviewed of biodiesel produced from microalgae, a prospective feedstock, is seven times higher than the current price of diesel, although much higher cost estimates have been reported. While significant cost reductions are needed for both types of second generation biofuels, the critical barriers are at different steps of the production process.

For cellulosic ethanol, the biomass conversion costs needs to be reduced. On the other hand, feedstock cost is the main issue for biodiesel. At present, policy instruments, such as fiscal incentives and consumption mandates have in general not differentiated between the first and second generation biofuels except in the cases of the US and EU. The policy regime should be revised to account for the relative merits of different types of biofuels. These new sensors will complement the firstgeneration medium-resolution pushbroom and high-definition video cameras that were mounted on the Russian segment of the ISS in early The Canadarm will then be used to install the remote sensing platform onto a CBM Common Berthing Mechanism hatch on Node 3, allowing the sensor electronics to be accessible from the inside of the station, thus limiting their exposure to the space environment and allowing for future capability upgrades.

The UrtheCast second-generation system will be able to take full advantage of the strengths that each of the individual sensors offers, such that the data exploitation capabilities of the combined sensors is significantly greater than from either sensor alone.

This represents a truly novel platform that will lead to significant advances in many other Earth Observation applications such as environmental monitoring, energy and natural resources management, and humanitarian response, with data availability anticipated to begin after commissioning is completed in early The objectives of this activity are: The work plan for this project activity consists of three major tasks: Definition of expected worst-case environments humidity, liquid composition and temperature at waste package outer surfaces as a function of time, and comparison with environments defined in the YMP baseline; Task 2.

Oxidation and corrosion tests of proposed second-generation outer container material; and Task 3. Second Generation waste package thermal analyses. Full funding was not provided for this project activity. A Second Generation Mobile Robot. Insight into biases and sequencing errors for amplicon sequencing with the Illumina MiSeq platform. The platform is manageable and affordable even for smaller labs.

This enables quick turnaround on a broad range of applications such as targeted gene sequencing , metagenomics, small genome sequencing and clinical molecular diagnostics. However, Illumina error profiles are still poorly understood and programs are therefore not designed for the idiosyncrasies of Illumina data. A better knowledge of the error patterns is essential for sequence analysis and vital if we are to draw valid conclusions. Studying true genetic variation in a population sample is fundamental for understanding diseases, evolution and origin.

We tested state-of-the-art library preparation methods for amplicon sequencing and showed that the library preparation method and the choice of primers are the most significant sources of bias and cause distinct error patterns. A " second generation " of ministry leadership.

Catholic health care leaders differ from others in the field in that "they are expected to serve as Jesus served, teach as Jesus taught, and lead as Jesus led, in order to heal as Jesus healed. This first generation was privileged in that it was tutored by and worked alongside women and men religious. Those religious are now mostly gone from the ministry, and that first generation of lay leaders will also be retiring in the not too distant future.

Leadership will then pass to a " second generation ," laypeople who have not worked alongside religious. How is this new generation to learn "to heal as Jesus healed"? In its efforts to fill a position, the system first assembles a preferred-candidate profile, based on 15 competencies, including seven core competencies. CHE then employs a recruitment process based on behavioral event interviewing.

All involved stakeholders participate in the interviews. A second generation polymerization monomeric reactants PMR polyimdes matrix system PMR 2 was characterized in both neat resin and composite form with two different graphite fiber reinforcements. Three different formulated molecular weight levels of laboratory prepared PMR 2 were examined, in addition to a purchased experimental fully formulated PMR 2 precurser solution.

Isothermal aging of graphite fibers, neat resin samples and composite specimens in air at C were investigated. Humidity exposures at 65 C and 97 percent relative humidity were conducted for both neat resin and composites for eight day periods. Composites were fire tested on a burner rig developed for this program. Results indicate that neat PMR 2 resins exhibit excellent isothermal resistance and that PMR 2 composite properties appear to be influenced by the thermo-oxidative stability of the reinforcing fiber.

Progress toward Brazilian cesium fountain second generation. The operation of a Cesium fountain primary frequency standard is strongly influenced by the characteristics of two important subsystems. The first is a stable frequency reference and the second is the frequency-transfer system. A stable standard frequency reference is key factor for experiments that require high accuracy and precision.

The frequency stability of this reference has a significant impact on the procedures for evaluating certain systematic biases in frequency standards. This paper presents the second generation of the Brazilian Cesium Fountain Br-CsF through the opto-mechanical assembly and vacuum chamber to trap atoms. We used a squared section glass profile to build the region where the atoms are trapped and colled by magneto-optical technique.

The opto-mechanical system was reduced to increase stability and robustness. This newest Atomic Fountain is essential to contribute with time and frequency development in metrology systems. AC application of second generation HTS wire. It can be engineered with a high degree of flexibility to manufacture practical 2G conductors with architectures and properties tailored for specific applications and operating conditions. For ac applications conductor and coil design can be geared towards low hysteretic losses.

For applications which experience high frequency ac fields, the stabilizer needs to be adjusted for low eddy current losses. For these applications a stainless-steel laminate is used. An example is a Low Pass Filter Inductor which was developed and built in this work. Methodology toward second generation expert systems. So-called First Generation Expert Systems were aimed at capturing the expert's know-how. Though providing remarkable achievements, this first wave did not give the expected outcome.

A new generation is getting out from the laboratories. Instead of remaining at a shallow level of knowledge - that is the unmotivated reasoning processes expressed by an expert when he is forced to tell them - one attempts to re-build this level of knowledge from the first principles which constitute the basis of an expert's knowledge. These systems are called deep knowledge-based, or second generation expert systems. Discussion in the three first parts rests on two examples: A first generation and a half system for process control in nuclear powers plants, than the system EXTRA for alarm processing in nuclear plants, wherein fonctional knowledge is explicitely represented.

We show how deep knowledge can be implemented, and the advantages that can be expected from this methodology. Qualitative Physics is discussed in the next part. Future research developments as well as potential payoffs are mentioned [fr. Medical Ultrasound Imaging is widely used clinically because of its relatively low cost, portability, lack of ionizing radiation, and real-time nature.

However, even with these advantages ultrasound has failed to permeate the broad array of clinical applications where its use could be of value. A prime example of this untapped potential is the routine use of ultrasound to guide intravenous access. In this particular application existing systems lack the required portability, low cost, and ease-of-use required for widespread acceptance.

Our team has been working for a number of years to develop an extremely low-cost, pocket-sized, and intuitive ultrasound imaging system that we refer to as the "Sonic Window. Through a high degree of custom front-end integration combined with multiplexing down to a 2 channel PC based digitizer this system acquired a full set of RF data over a course of transmit events. While initial results were encouraging, this system exhibited limitations resulting from low SNR, relatively coarse array sampling, and relatively slow data acquisition.

We have recently begun assembling a second-generation Sonic Window system. This system uses a element fully sampled array operating at 5. We present initial results from this front-end and present benchmark results from a software beamformer implemented on the Analog Devices BF DSP. We discuss our immediate plans for further.

A second-generation supersonic transport. This is of particular importance with respect to support technology such as airport terminals and runways. Based on the above introductory remarks, a possible approach to the design of a second-generation supersonic civil transport is presented here. The design Mach number for this aircraft is 3. This value was chosen as it represents the limiting Mach number in the absence of active cooling. The ensuing design attempts to deal with the particular problems that are the most demanding, while relying on proven technology where it is adequate.

The report clearly does not solve, or even deal with, every aspect of the aircraft design. Rather, a general direction is suggested and supported with initial, approximate calculations. A second generation genetic map for rainbow trout Oncorhynchus mykiss. Full Text Available Abstract Background Genetic maps characterizing the inheritance patterns of traits and markers have been developed for a wide range of species and used to study questions in biomedicine, agriculture, ecology and evolutionary biology. The status of rainbow trout genetic maps has progressed significantly over the last decade due to interest in this species in aquaculture and sport fisheries, and as a model research organism for studies related to carcinogenesis, toxicology, comparative immunology, disease ecology, physiology and nutrition.

We constructed a second generation genetic map for rainbow trout using microsatellite markers to facilitate the identification of quantitative trait loci for traits affecting aquaculture production efficiency and the extraction of comparative information from the genome sequences of model fish species. Results A genetic map ordering microsatellite loci spanning a sex-averaged distance of Microsatellite markers, representing pairs of loci resulting from an evolutionarily recent whole genome duplication event, identified duplicated regions within the rainbow trout genome.

Microsatellites associated with genes through expressed sequence tags or bacterial artificial chromosomes produced comparative assignments with tetraodon, zebrafish, fugu, and medaka resulting in assignments of homology for loci. Conclusion The second generation NCCCWA genetic map provides an increased microsatellite marker density and quantifies differences in recombination rate between the sexes in outbred populations.

It has the potential to integrate with cytogenetic and other physical maps, identifying paralogous regions of the rainbow trout genome arising from the evolutionarily recent genome duplication event, and. The second generation of biofuels aims at ; La seconde generation de biocarburants vise Research work is intensifying on the second generation of biofuels in order to optimize the processes and to reduce the production costs. Poorly valorized vegetable resources are targeted in order to avoid conflicts with other agricultural processes.

Two ways are explored since several years: So far, no method allows to recover oils with a satisfactory energy efficiency but several pilot-plants are under study or already in operation Futurol, BTL biomass-to-liquid.. The advent of DNA sequencing technologies and the various applications that can be performed will have a dramatic effect on medicine and healthcare in the near future. There are several DNA sequencing platforms available on the market for research and clinical use.

Based on the medical laboratory scientist or researcher's needs and taking into consideration laboratory space and budget, one can chose which platform will be beneficial to their institution and their patient population. Although some of the instrument costs seem high, diagnosing a patient quickly and accurately will save hospitals money with fewer hospital stays and targeted treatment based on an individual's genetic make-up. By determining the type of disease an individual has, based on the mutations present or having the ability to prescribe the appropriate antimicrobials based on the knowledge of the organism's resistance patterns, the clinician will be better able to treat and diagnose a patient which ultimately will improve patient outcomes and prognosis.

A platform -independent method for detecting errors in metagenomic sequencing data: It also provides global whole sample error estimates that can be used to identify samples with high or varying levels of sequencing error that may confound downstream analyses, particularly in the case of studies that utilize data from multiple sequencing samples.

For shotgun metagenomic data, we believe that DRISEE provides estimates of sequencing error that are more accurate and less constrained by technical limitations than existing methods that rely on reference genomes or the use of scores e. We present DRISEE as an open-source, platform -independent method to assess sequencing error in shotgun metagenomic data, and utilize it to discover previously uncharacterized error in de novo sequence data from the and Illumina sequencing platforms.

A tale of three next generation sequencing platforms: The pace of change in this area is rapid with three major new sequencing platforms having been released in Here we compare the results obtained with those platforms to the performance of the Illumina HiSeq, the current market leader. In order to compare these platforms , and get sufficient coverage depth to allow meaningful analysis, we have sequenced a set of 4 microbial genomes with mean GC content ranging from Together, these represent a comprehensive range of genome content.

Here we report our analysis of that sequence data in terms of coverage distribution, bias, GC distribution, variant detection and accuracy. We analysed the ability to call variants from each platform and found that we could call slightly more variants from Ion Torrent data compared to MiSeq data, but at the expense of a higher false positive rate. Variant calling from Pacific Biosciences data was possible but higher coverage depth was required. Conclusions All three fast turnaround sequencers evaluated here were able to generate usable sequence.

However there are key differences between the quality of that data and the applications it will support. The data generated was largely comparable between sequencing platforms Although we acknowledge our analyses were limited to animal material, our observations suggest that the BGISEQ holds the potential to represent valid and potentially valuable alternative platform for palaeogenomic data generation, that is worthy of future exploration by those interested Use of four next-generation sequencing platforms to determine HIV-1 coreceptor tropism.

In this study, HIV-1 co-receptor usage was predicted for twelve patients scheduled to start a maraviroc-based antiretroviral regimen. Cross- platform variation was evaluated, including number of reads, read length and error rates. Each platform detected all major virus variants within the HIV-1 population with similar frequencies. Identification of non-R5 viruses was comparable among the four platforms , with minor differences attributable to the algorithms used to infer HIV-1 tropism. In conclusion, all four NGS platforms were able to detect minority non-R5 variants at comparable levels suggesting that any NGS-based method can be used to predict HIV-1 coreceptor usage.

Europe and the United States. The public debate about the second generation in Europe has taken a dramatic shift in the last five years. The riots in the banlieues in France, involving mostly Algerian and Moroccan second-generation youth, pitched the cherished republican model into deep crisis.

In the Netherlands, arguments.

A parallel reconfigurable platform for efficient sequence alignment Bioinformatics is one of the emerging trends in today's world. Look for the Kindle MatchBook icon on print and Kindle book detail pages of qualifying books. Print edition must be purchased new and sold by Amazon. Gifting of the Kindle edition at the Kindle MatchBook price is not available. Learn more about Kindle MatchBook. Kindle Cloud Reader Read instantly in your browser. Editorial Reviews About the Author Having vocally predicted the recent credit crisis while the Manager of Oversight for Complex Instruments at Indymac Bank, Jake Shannon also accurately warned of the current deflation we are experiencing in his Anomaly: Revolutionary Knowledge In Everyday Life.

The Hijacking of Liberty in America. Jake Shannon earned his M. He lives in Salt Lake City with his wife and children. Product details File Size: September 12, Sold by: Share your thoughts with other customers. Write a customer review. Showing of 2 reviews. Top Reviews Most recent Top Reviews. There was a problem filtering reviews right now.

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Please try again later. Reviewing this one is tough - it ain't entirely digested yet, and I'm in the midst of a re-read - but it wants a review, so here I am. This should have been several pages longer, from a pure style perspective. There's a heck of a lot of information presented, but the author seems to want to get it all across with the absolute minimum verbiage - and a bit more explanation for the average reader would have improved it.

A central argument was made, but not in an entirely convincing fashion - the author claims that US Federal Reserve Notes are in a contraction, and are being deflated - and he has reasons for thinking so, and cites them well. Studying Prophecy Can Change Your Life Strengthen your faith and find real hope for the future in this extensive resource that provides concise answers to your most burning ….

This ebook deals with a profoundly decisive change in world history. There's going to be a transition from governance under fallen man to a government from God Himself. It moves on to examine the responses of both the dispossessed …. From religious tomes to current folk prophesies, recorded history reveals a plethora of narratives predicting or showcasing the end of the world.

The incident at Waco, the subway ….